Darshit Shah
Senior Scientist Regeneron Pharmaceuticals
Seminars
Thursday 23rd July 2026
Unveiling the Shadows: How We Can Harness Proteogenomics Techniques to Uncover Hidden Antigens
10:00 am
- Highlight the integrated approach combining Ribosome Profiling (Ribo-Seq), RNA sequencing, and mass spectrometry to identify novel "dark antigens" – previously unannotated proteins derived from non-coding RNAs, alternative reading frames, and alternative splicing events
- Emphasize how this approach reveals tumor-specific cell surface targets missed by traditional genomic approaches, focusing on the workflow that identifies transmembrane domain-containing proteins with tumor-specific expression Outline the systematic approach for target validation, starting with computational prediction and progressing through experimental confirmation of expression and localization
- Outline the systematic approach for target validation, starting with computational prediction and progressing through experimental confirmation of expression and localization
